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KMID : 0364820170530010009
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Korean Journal of Microbiology
2017 Volume.53 No. 1 p.9 ~ p.19
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A comparison of community structure and denitrifying ratio for denitrifying bacteria dependent on agricultural methods and seasons
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Yoon Jun-Beom
Park Kyeong-Ryang
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Abstract
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We studied soil composition, N2O production, a number of denitrifying bacteria, community structure and T-RFLP patterns of denitrifying bacteria dependent on agricultural methods with the change of seasons. Analyses of the soil chemical composition revealed that total carbon and total organic carbon contents were 1.57% and 1.28% in the organic farming soil, 1.52% and 1.24% in the emptiness farming soil, and 1.40% and 0.95% in traditional farming soil, respectively. So, the amount of organic carbon was relatively high in the environment friendly farming soils than traditional farming soils. In case of N2O production, the amount of N2O production was high in May and November soils, but the rate of N2O production was fast in August soil. The average number of denitrifying bacteria were 1.32 × 104 MPN · g-1 in the organic farming soil, 1.17 × 104 MPN · g-1 in the emptiness farming soil, and 6.29 × 103 MPN · g-1 in the traditional farming soil. It was confirmed that the environment friendly farming soil have a larger number of denitrifying bacteria than the traditional farming soil. As a result of the phylogenetic analyses, it was confirmed that six clusters were included in organic farming soil among total 10 clusters. And the result of PCA profile distribution of T-RFLP pattern on agricultural methods, the range of distribution showed wide in the organic farming method, relatively narrow in the conventional farming method, and middle in the emptiness farming method. Therefore, we could concluded that the distribution and the community structure of denitrifying bacteria were changed according to the agricultural methods and seasons.
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KEYWORD
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agricultural methods, community structure, denitrifying bacteria, soil composition, T-RFLP
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